[et_pb_section fb_built=”1″ fullwidth=”on” _builder_version=”4.16″ use_background_color_gradient=”on” background_color_gradient_stops=”#ffffff 0%|#313476 100%” background_color_gradient_start=”#ffffff” background_color_gradient_end=”#313476″ locked=”off” global_colors_info=”{}”][et_pb_fullwidth_image src=”https:\/\/www.aquabol.sk\/cesamir2020\/wp-content\/uploads\/2023\/11\/CESAMIR-banner-NEW01.png” title_text=”CESAMIR banner NEW01″ _builder_version=”4.16″ background_color=”#93acc1″ use_background_color_gradient=”on” background_color_gradient_stops=”#2b87da 0%|rgba(239,255,249,0.11) 100%” background_color_gradient_end=”rgba(239,255,249,0.11)” global_colors_info=”{}”][\/et_pb_fullwidth_image][\/et_pb_section][et_pb_section fb_built=”1″ custom_padding_last_edited=”off|desktop” admin_label=”hero section” _builder_version=”4.16″ use_background_color_gradient=”on” background_color_gradient_stops=”#ffffff 0%|#e7edf9 100%” background_color_gradient_start=”#ffffff” background_color_gradient_end=”#e7edf9″ custom_margin=”|||” custom_padding=”50px||50px|||” custom_padding_tablet=”130px||130px|” global_colors_info=”{}”][et_pb_row column_structure=”3_5,2_5″ _builder_version=”4.16″ custom_margin=”|auto|54px|auto||” custom_padding=”10px|0px|10px|0px” border_color_all=”#4646c4″ global_colors_info=”{}”][et_pb_column type=”3_5″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.23.1″ text_font=”||||||||” header_font=”||||||||” header_2_font=”|600|||||||” header_2_font_size=”36px” header_2_line_height=”1.4em” animation_style=”fade” global_colors_info=”{}”]<\/p>\n
[\/et_pb_text][et_pb_divider color=”#4496da” divider_position=”center” divider_weight=”3px” _builder_version=”4.23.1″ max_width=”90px” max_width_tablet=”13%” max_width_last_edited=”off|desktop” custom_margin=”15px|||” custom_padding=”|||” animation_style=”fade” global_colors_info=”{}”][\/et_pb_divider][\/et_pb_column][et_pb_column type=”2_5″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_image src=”https:\/\/www.aquabol.sk\/cesamir2020\/wp-content\/uploads\/2019\/10\/Day01a.png” align_tablet=”center” align_phone=”” align_last_edited=”on|desktop” admin_label=”illustration” _builder_version=”4.23.1″ transform_translate=”-3px|0px” transform_translate_linked=”off” width=”90.3%” custom_margin=”3px|||||” custom_padding=”2px||1px|||” filter_hue_rotate=”285deg” filter_saturate=”120%” animation_style=”slide” animation_direction=”right” animation_intensity_slide=”10%” global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.25.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.25.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_toggle title=”9:00 PLENARY: N\u00faria Bonada – Freshwater biodiversity in Mediterranean climate regions: current status and future trends” open_toggle_text_color=”#0C71C3″ closed_toggle_text_color=”#0C71C3″ icon_color=”#313476″ use_icon_font_size=”on” icon_font_size=”25px” open_icon_color=”#313476″ open_use_icon_font_size=”on” open_icon_font_size=”25px” _builder_version=”4.25.2″ title_text_color=”#0C71C3″ title_font=”|600|||||||” title_text_align=”center” title_font_size=”22px” closed_title_font=”||||||||” closed_title_text_align=”center” closed_title_line_height=”1.8em” body_font_size=”20px” body_line_height=”1.8em” background_color=”#f5f9fb” text_orientation=”center” custom_margin=”-63px||||false|false” custom_padding=”||29px|||” global_colors_info=”{}”]<\/p>\n
Freshwater ecosystems account for 0.3% of the planet’s freshwater but they are the\u00a0habitat for 9% of all described species and 35% of vertebrate species. The levels of\u00a0freshwater biodiversity loss are alarming, doubling those found in terrestrial or marine ecosystems. Mediterranean climate regions are considered global hotspots of\u00a0biodiversity, also for freshwater organisms. Rivers in these regions (med-rivers) are unique ecosystems because of their predictable winter flooding and summer drought regimes. They support many species adapted to both floods and droughts, and their high levels of freshwater biodiversity are explained by past historical events and current environmental heterogeneity. At the same time, Med-rivers have been affected for\u00a0centuries, in some cases millennia, by multiple human activities that increasingly threaten their biodiversity. These threats include changes in land use, nutrient loads, heavy metal concentrations, salinity, water withdrawals, invasive species and, more recently, xenobiotics or emerging organic pollutants. In addition, future climate change scenarios predict increases in drought conditions and in the occurrence of extreme events, such as floods, heat waves, and wildfires. The diversity of freshwater organisms is\u00a0declining more rapidly in med-rivers than in rivers anywhere else in the world and, for\u00a0some taxonomic groups, Mediterranean regions have more introduced than native species. Freshwater biodiversity conservation in med-rivers requires innovative approaches to account for both natural and human disturbances. Current protection figures, including the Natura\u00a02000 network, do not appear to be very efficient in\u00a0protecting freshwater biodiversity, and current methods to assess the ecological status fail when applied to characteristic med-rivers. To ensure the protection of\u00a0freshwater biodiversity and ecosystems in Mediterranean climate regions, the\u00a0adaptation and development of specific management protocols and actions to\u00a0the\u00a0characteristics of med-rivers is needed, specially under future climate scenarios.<\/span><\/p>\n [\/et_pb_toggle][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.16″ custom_padding=”10px|0px|40px|0px||” border_color_all=”#4646c4″ global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_accordion icon_color=”#0C71C3″ use_icon_font_size=”on” icon_font_size=”25px” _builder_version=”4.25.2″ _module_preset=”default” width=”100%” max_width=”100%” module_alignment=”center” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][et_pb_accordion_item title=”RS1: DIVERSITY – BIOGEOGRAPHY – PHYLOGEOGRAPHY 1″ open=”on” _builder_version=”4.25.2″ _module_preset=”default” body_font=”|600|on||||||” body_text_align=”left” custom_margin=”||5px||false|false” global_colors_info=”{}” toggle_text_color=”#0C71C3″ toggle_level=”h5″ toggle_font=”|700|||||||” toggle_text_align=”left” toggle_font_size=”20px”]<\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”10:30 Podwysocki K: Environment, phylogeographic lineages or ranges \u2013 what drives the high morphological variation of the successful invader, Dikerogammarus villosus (Sovinsky, 1894) in European aquatic ecosystems?” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” custom_margin=”||5px||false|false” hover_enabled=”0″ global_colors_info=”{}” toggle_level=”h5″ toggle_font=”–et_global_heading_font|500|||||||” toggle_text_align=”left” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n Phenotypic diversity plays a crucial role in facilitating the establishment and expansion of invasive populations in new environments. Particularly high levels of plasticity can be\u00a0observed among the diverse Ponto-Caspian fauna that invaded inland European waters. However, the precise influence of the environment on shaping the phenotypic variation of these successful invaders remains inadequately understood. A good model species for such studies is Dikerogammarus villosus<\/i> (Sovinsky, 1894) (Gammaridae, Amphipoda). This highly predatory and voracious species invaded a notable part of\u00a0Europe within a few decades. It originated in the Ponto-Caspian region and the two isolated populations from the Danube and the Dnieper deltas colonised Europe through two phylogenetic lineages \u2013 the Western (via Danube) and the Eastern (via Dnieper). Although this species has a broad distribution and is currently expanding in\u00a0Europe, its phenotypic diversity related to traits that determine invasive success was not studied. Therefore, we aimed to test morphological variation of functional traits that directly or indirectly reflect the diet of D.\u00a0villosus<\/i> across the European continent. Our results show that D.\u00a0villosus<\/i> exhibits a high morphological variability across Europe and\u00a0that it is mainly shaped by the type of environment. We discovered an\u00a0enhancement of predation-related traits in brackish waters compared to freshwaters. These results suggest that D.\u00a0villosus<\/i> could exert a greater threat on macroinvertebrate communities in brackish environments. Morphological differentiation among phylogenetic lineages and native vs. invaded ranges is weaker and mainly concerns locomotion and food processing traits (i.e., mouthparts and stomachs). Both lineages display a unique pattern of morphospace change between native and invaded ranges. A strong morphospace expansion in the invaded range of the Eastern Lineage suggests a higher plasticity of this lineage and possibly higher success in establishment in new habitats. Our results highlight the importance of accounting for ecological, evolutionary, and biogeographical factors when studying the phenotypic diversity of invasive species at broad continental scales. The results will be an important input to understanding the\u00a0invasion dynamics of Ponto-Caspian amphipods in Europe.<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”10:50 Fics\u00f3r M: Hydropsyche in the multiverse of rivers \u2013 a multi-scale, multi-model ensemble approach to explain the longitudinal distribution of larval net-spinning caddisfly species-groups” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” custom_margin=”||5px||false|false” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n Riverine ecosystems are dendritic, linear networks that are connected longitudinally, laterally, and vertically. Environmental gradients, as a result of uni-directional flow, create a great number of diverse but still interconnected microhabitats, making stream ecosystems the most heterogeneous, most complex, hierarchically nested systems where ecological processes both in the upstream reaches and the adjacent catchments govern local conditions. Examining, explaining, and especially modelling the\u00a0relationships between riverine species and their environment are therefore particularly challenging and require a multi-scale and multi-model approach.<\/span><\/p>\n In the present study, our aims were to build an ensemble of base learner machine learning (ML) models based on selected multi-scale environmental parameters shaping the sequential distribution of ten Central European species of the genus Hydropsyche<\/i> that could explain and effectively predict the occurrence of species and\/or groups of\u00a0them with similar ecological preferences.<\/span><\/p>\n Our results show that several gradient-like and pollution related factors that are usually measured as part of monitoring programmes (such as those of Water Framework Directive) at finer spatial scales and in higher detail could be considered suitable to\u00a0complement or substitute those (e.g., topographic, geographic or precipitation data) that are routinely included in species distribution models (SDMs). It is also revealed that an ensemble of multi-scale models can outperform single modelling algorithms in\u00a0mapping the longitudinal distribution of species and species groups.<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”11:10 S\u0142omczy\u0144ski K: Diversity of non-biting midges (Chironomidae) inhabiting common water moss (Fontinalis antipyretica Hedw.) microhabitats \u2013 a case study from river Rawka (central Poland)” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” custom_margin=”||5px||false|false” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n Vegetation in the riverine ecosystems plays a crucial role in providing food and shelter for the invertebrates. Not only the vascular plants but also the aquatic mosses are important habitats for the benthos. The common water moss (Fontinalis antipyretica<\/i> Hedw.) occurrence is limited by a presence of solid substrates, e.g., stones or submerged wood to which plant can attach. F.\u00a0antipyretica<\/i> occurs in highly oxygenated and clear rivers. Therefore, the common water moss as the microhabitats for Chironomidae in the lowland river Rawka (central Poland) was studied. Rawka is protected as a nature reserve, being one of the few natural rivers of the Polish Lowlands. Its benthic assemblages have not been studied extensively so far, only preliminary research conducted indicates that Chironomidae are the least-known and most underestimated group. As the riverbed of\u00a0the Rawka is not regulated or dredged, it exhibits a variety of moss microhabitats. The\u00a0material was collected from 22 sites, including samples of Chironomidae larvae and\u00a0algal periphyton, and a control sample from the bottom. The physicochemical conditions have been examined. As the diatoms are the main component of periphytic invertebrates\u2019 diet, their species composition and richness were analysed. The preliminary results indicate 20 species of Chironomidae, including 3 new species to the Polish fauna. Most of the recorded species are rheobiontic and small-sized, i.e., Rheotanytarsus photophilus<\/i>, Eukiefferiella claripennis<\/i> and Thienemanniella majuscula<\/i>. Eighteen Chironomidae species have been recorded for the first time from this river which leads to\u00a0the hypothesis that aquatic moss microhabitats hide a substantial diversity of\u00a0Chironomidae. The new data contribute to the knowledge concerning the aquatic fauna of Rawka Nature Reserve and shed new light on aquatic moss as an important habitat for macroinvertebrates.<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”11:30 Drenov\u00e1cz M: Fantastic beetles and where to find them? Do spring floods govern the occurrence of Graphoderus bilineatus in Hungary?” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n The Graphoderus bilineatus<\/i>, a highly protected species listed as Vulnerable (VU) by\u00a0the\u00a0IUCN Red List and designated under Natura\u00a02000 in Hungary, holds significant conservation importance at both national and European levels. Despite increasing literature on its ecology, knowledge gaps persist, as the life history, development, and\u00a0ecological preferences of the G.\u00a0bilineatus<\/i> exhibit considerable variability across regions and over time. Understanding these variations is crucial for developing effective long-term conservation strategies targeting both the species and its habitats. Decades of\u00a0field observations in Hungary have revealed a consistent pattern: the abundance and\u00a0presence of G.\u00a0bilineatus<\/i> are closely linked to spring floods, particularly within floodplain habitats, a phenomenon less observed in other European countries to\u00a0the\u00a0north. We quantified the time intervals between the occurrence of spring floods and the presence or absence of the species in known habitats along the Drava, Danube, Bodrog, and Tisza rivers. Our findings demonstrate a significant correlation between the\u00a0occurrence and appearance of the species and spring floods. Furthermore, our data suggest that these floods may act as a triggering mechanism, prompting the G.\u00a0bilineatus<\/i> to initiate flight and potentially colonise new water bodies within its range. Understanding the interplay between spring flood dynamics and the ecology of G.\u00a0bilineatus<\/i> is essential for informing targeted conservation efforts aimed at preserving this species and its associated habitats.<\/span><\/p>\n [\/et_pb_accordion_item][\/et_pb_accordion][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_accordion icon_color=”#0C71C3″ use_icon_font_size=”on” icon_font_size=”25px” _builder_version=”4.25.2″ _module_preset=”default” width=”100%” max_width=”100%” module_alignment=”center” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][et_pb_accordion_item title=”SS2: RESPONSE TO DRYING 1″ open=”on” _builder_version=”4.25.2″ _module_preset=”default” body_font=”|600|on||||||” body_text_align=”left” custom_margin=”||5px||false|false” global_colors_info=”{}” toggle_text_color=”#0C71C3″ toggle_level=”h5″ toggle_font=”|700|||||||” toggle_text_align=”left” toggle_font_size=”20px”]<\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”10:30 Pa\u0159il P: Dry stream channels as attractive habitats for multiple biotic groups” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” custom_margin=”||5px||false|false” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n Current trajectories of climate change are manifested in the river networks of Central Europe by dramatic shifts from a perennial to an intermittent flow regime. Decrease of\u00a0annual discharge is also associated with remarkable seasonal shifts in the flow duration, when first dry episodes can occur even in early spring (April-May) and could last until the\u00a0cold season in winter (December-January) with the flow absence over half a year. Such prolonged dry episodes offer an extended time-window for stream colonisation by many terrestrial groups, which originally use habitats surrounding river channels. Newly occurred biotope of dry riverbed could provide an attractive environment (e.g., exposed sandy and\u00a0gravel bars), that is not available in regulated rivers and anthropogenically modified floodplains.<\/span><\/p>\n Effects of stream drying on aquatic biota were more intensively studied in the last decade also in temperate Central Europe; however, interactions between terrestrial and\u00a0aquatic groups are still poorly examined. Nevertheless, we can expect multiple impacts of invading terrestrial fauna and flora on aquatic biota during the dry phase (e.g., predation in pools and\u00a0dry bottom, streambed ploughing, colonisation by\u00a0terrestrial plants, etc.). Additionally, some of these effects could persist and\u00a0consequently affect aquatic communities after the\u00a0flow resumption. We show some examples of exploitation of dry channels by terrestrial groups, which could affect aquatic biota such as macroinvertebrates and fish (e.g., predation by terrestrial invertebrates and large vertebrates as wild boars or piscivorous birds). Moreover, some terrestrial invertebrates and plants including endangered taxa could recognise dry riverbed as attractive habitat, which enable them successful reproduction and\u00a0expansion of populations. Such long-time dry reaches with sufficient light exposition are also rapidly colonised by terrestrial vascular plants, which could later offer food resources and shelter for aquatic macroinvertebrates in the early stages of\u00a0recolonisation after rewetting.<\/span><\/p>\n Contrary to our expectations, we also recorded a few examples of an active movement of\u00a0strictly aquatic stages or macroinvertebrates, which migrate not only within dry channels, but also actively immigrate to terrestrial riparian habitats several meters outside the channel. These findings shed a new light on the role of currently spreading habitats of intermittent streams, which emphasise an urgent need of their examination during progressing climate changes in the temperate zone of Central Europe. Research was supported by the projects H2020 DRYVER (869226) and TACR project SS06010258.<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”10:50 H\u00e1rs\u00e1gyi D: Navigating through drying histories: switching roles of connectivity in Trichoptera metacommunity organisation” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” custom_margin=”||5px||false|false” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n Climate change induces unprecedented dry periods in previously perennial streams, significantly impacting aquatic macroinvertebrate metacommunities. We assumed that the historical (i.e., several dozens of years) patterns of presence or absence of drying in dry river networks (DRNs) modifies the relative importance of local and regional, spatial, and niche-related environmental factors in metacommunity organisation. In this study, we sampled Trichoptera metacommunities in three Drying River Networks (DRNs) along a latitudinal gradient across three countries, also representing a drying gradient. In Croatia, drying has been present in the DRN for a very long time, with an almost constant annual occurrence. At the other end of the scale is the Hungarian DRN, where the drying has appeared in recent years and with varying spatial and temporal annual intensity, but is becoming more and more intense, while the drying history of the Czech DRN lies between these two with highly unpredictable occurrences. Here, we hypothesised that the relevance of spatio-temporal connectivity differs according to the historical drying patterns in each catchment. In 2021, the Trichoptera metacommunities were sampled six times bimonthly at 20\u201325 sites per DRN, and a comprehensive set of local and regional environmental parameters were recorded. Spatial variables were generated through Moran\u2019s eigenvector maps (MEM) and asymmetric eigenvector maps (AEM) from watercourse distances among sites in each river network. Spatio-temporal connectivity was assessed using the STcon framework, and values were calculated for different time frames (for 30, 200, 365, 730, 1825, and 3650 days before sampling). We analysed the relative relevance of spatial (MEM and AEM), niche, and spatio-temporal (STcon) drivers of these communities by means of hierarchical variance partitioning. The explanatory power of the STcon was the highest in the Hungarian DRN and the lowest in Croatia, with significant differences observed across different time frames. In the Hungarian DRN, the connectivity calculated for time frames shorter than one year proved to be the most relevant factor, while in the case of the Croatian DRN, the five- and ten-year-long connectivity hold the greatest explanatory power. These findings underscore the pivotal role of spatio-temporal connectivity in shaping metacommunities, albeit with varying impacts across river basins characterised by different drying histories. Such insights are critical for understanding and managing the ecological ramifications of climate-induced alterations in aquatic ecosystems. This work was supported by the EU-funded DRYvER project (869226).<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”11:10 Sychra J: Research of aquatic invertebrate communities in ephemeral wetlands on arable land” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” custom_margin=”||5px||false|false” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n Temporary wetlands belong to the most threatened ecosystems within Europe inhabited by a unique fauna of aquatic invertebrates. However, we still do not have much information about the main determinants of the composition and diversity of their communities. We have sampled altogether 36 ephemeral field wetlands in southern Moravia (SE part of the Czech Republic), which periodically appear on arable land after heavy rains or snow melting. To evaluate the seasonal aspect, sites were sampled after flooding in spring and summer. Non-biting midges (Chironomidae), aquatic beetles (Coleoptera), water bugs (Heteroptera), and true flies (Diptera) were the most dominant taxa. Field wetlands varied significantly between seasons in algal cover, water temperature, and some chemical characteristics of water. Both higher numbers of species and higher abundances of invertebrates were found during the summer season. Large branchiopods (Branchiopoda: Anostraca, Notostraca, Spinicaudata) were the main drivers of variability in community composition during the summer season, with increasing water turbidity, and decreasing numbers of species and abundance of other invertebrates as\u00a0their numbers increased. Among the environmental variables, seasonality, turbidity, and water temperature contributed most to the variability in the community composition. Another important factor influencing the colonisation of these temporary habitats is the presence of seed banks. Based on zooplankton sampling, we found a significant positive influence of the continuity and history of sites on species diversity and the occurrence of\u00a0regionally rare species. Compared to other standing water habitats, ephemeral field wetlands host unique communities of aquatic invertebrates, including several protected and endangered species, and serve as the last remnants of the landscape suitable for the\u00a0occurrence of these unique communities. This is also why they deserve attention and\u00a0protection, especially against targeted drainages or conversion into permanent water bodies with the subsequent appearance of fish. Our results represent one of the first insights into the diversity and functioning of aquatic invertebrate communities in\u00a0the\u00a0unique habitats of ephemeral field wetlands in central Europe.<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”11:30 Loskotov\u00e1 B: Role of invertebrate dispersal pathways in intermittent streams recolonisation after rewatering \u2013 an in situ experimental study” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n The dispersal abilities of aquatic organisms and their recolonisation abilities are generally underexplored in major biotic groups. This deficiency is a principal obstacle to\u00a0understanding dispersal as a regional process structuring ecological communities, especially in spatiotemporally dynamic intermittent streams. Freshwater invertebrates are commonly spread inhabitants of inland aquatic systems, characterised as frequent, widespread dispersers able to reflect environmental conditions and their changes with a\u00a0fast response at the species, population, or community level. Stream intermittency represents a hydrological disturbance acting as an environmental filter providing intense selection pressure on instream biota \u2013 it significantly regulates population and community structure and dynamics, particularly in the current period of accelerated climate change.<\/span><\/p>\n We tried to experimentally examine different recolonisation pathways used by benthic macroinvertebrates in terms of both taxonomic and functional community composition and evaluate their potential for successful community recovery after the drying period, expressed in total abundance and biomass. We considered four possible recolonisation pathways (i.e., downstream, upstream, hyporheic and aerial) with corresponding experimental units installed in situ, where only one of the mentioned recolonisation ways was allowed. Each treatment was represented by three replicates composed of wired boxes filled with an artificial gravel substrate exposed in a stream for two weeks.<\/span><\/p>\n Significant differences among treatments were evident in the alpha-diversity indices; Shannon’s diversity index was lowest in the hyporheic treatment, whereas the taxa richness and total abundance were lowest in the aerial treatment. Total biomass was highest in the hyporheic treatment, despite its lowest biodiversity. Two invertebrate groups, chironomids, and crustaceans dominated all the treatment types. The analysis of\u00a0species traits showed different metacommunity structures in each treatment. Hyporheic treatments were frequently populated by inhabitants preferring sandy and gravel substrates, or species typical for a hyporheic zone. Species with flexible life cycles predominantly colonised aerial treatments. In upstream treatments dominated species from lower stream zones or species typical for standing waters, whereas species from upper stream sections were less frequent.<\/span><\/p>\n While downstream drift was the most favourable pathway regarding biodiversity, the hyporheic pathway was the most important recolonisation way considering biomass. According to our study, the aerial and upstream recolonisation pathways have only a marginal contribution to community recovery after rewatering in a short-term horizon. The study was supported by the H2020 project DRYvER (869226).<\/span><\/p>\n [\/et_pb_accordion_item][\/et_pb_accordion][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.16″ custom_padding=”10px|0px|40px|0px||” border_color_all=”#4646c4″ global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_accordion icon_color=”#0C71C3″ use_icon_font_size=”on” icon_font_size=”25px” _builder_version=”4.25.2″ _module_preset=”default” width=”100%” max_width=”100%” module_alignment=”center” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][et_pb_accordion_item title=”RS1: DIVERSITY – BIOGEOGRAPHY – PHYLOGEOGRAPHY 2″ open=”on” _builder_version=”4.25.2″ _module_preset=”default” body_font=”|600|on||||||” body_text_align=”left” custom_margin=”||5px||false|false” global_colors_info=”{}” toggle_text_color=”#0C71C3″ toggle_level=”h5″ toggle_font=”|700|||||||” toggle_text_align=”left” toggle_font_size=”20px”]<\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”13:00 Hupa\u0142o K: Spatio-temporal niche partitioning drives a unique case of stable coexistence of two freshwater amphipods in Sicily” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” custom_margin=”||5px||false|false” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n Species coexistence has been one of the focal points in ecology. Particularly, the coexistence of closely related taxa occupying similar niches provides an interesting research topic. According to ecological theory, such an event is unlikely. However, recent evidence indicates that this phenomenon emerges across a wide range of\u00a0phylogenetically distinct taxa and from all types of habitats. Nevertheless, the stability and the mechanisms facilitating such coexistence remain unknown. Therefore, a\u00a0multitude of evidence is needed to better understand the dynamics and eco-evolutionary drivers underlying this ecological paradox. Here, we focused on a unique case on the island of Sicily where two local amphipod species, Homeogammarus sicilianus<\/i> and Homeogammarus adipatus<\/i>, co-occur in a single river system, Fosso del Tempio. Despite being widespread across the island, they solely co-occur in the studied system. This raises the question of whether this coexistence is stable and supported by\u00a0niche differentiation or reflects an unstable, possibly seasonal, co-occurrence. By applying an array of DNA-based methods and seasonal fine-scale sampling, we aimed to investigate potential niche differentiation by studying the microhabitat preferences, dietary habits, reproductive activity, and potential influence of parasites on the co-occurring amphipod species. Our results indicate that both species co-occur at nearly all studied sampling sites across the entire river system, regardless of the season. We also found significant differences in microhabitat distribution amongst both species, with H.\u00a0sicilianus<\/i> occurring more in biotic and H.\u00a0adipatus<\/i> found more in abiotic microhabitats. DNA metabarcoding results revealed different feeding strategies between the two species, with H.\u00a0adipatus<\/i> being a more specialised feeder and\u00a0H.\u00a0sicilianus<\/i> having a more generalistic diet. We also found differences in species\u2019 reproductive activity over the year. Finally, even though both species shared the same parasite lineages, the parasite composition and prevalence patterns significantly differed between the two host species. The obtained results support a stable coexistence between the two studied species. However, based on the observed niche differences, H.\u00a0sicilianus<\/i> seems better suited to outcompete H.\u00a0adipatus<\/i> in a longer evolutionary timescale, challenging the stability of observed coexistence. Our results indicate that a highly integrative framework is needed to disentangle drivers of species coexistence, supporting its use for studying similar cases.<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”13:20 Annal I: Spatial-temporal dynamic of syntopic cryptic species of the Gammarus fossarum complex (Amphipoda) and their ecological differentiation” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” custom_margin=”||5px||false|false” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n Gammarus fossarum<\/i>, a wide-ranging cryptic species complex, has exceptional diversity in the Western Carpathians, whereat local syntopy of distinct lineages is common. Previous research from Western Europe, as well as the Carpathians, has indicated that at\u00a0least some of G.\u00a0fossarum<\/i> lineages differ ecologically (e.g., in resistance to\u00a0anthropogenic pollution, or interactions with parasites). Within the Vset\u00edn region, Czechia, three highly divergent, reproductively isolated lineages of the complex, i.e., biological species, commonly co-occur. In this project, we explore the puzzling nature of\u00a0this coexistence, specifically to what extent the lineage composition is influenced by\u00a0the local conditions. Conveniently, many streams of the area exhibit a downstream environmental gradient, leading to a gradual change in conditions from forested headwaters to an urbanised stream mouth. We expect that local conditions promote dominance of certain lineages, and thus the compositions of the Gammarus<\/i> communities change gradually along the stream path. We hypothesise that this pattern should be consistent between streams, and temporally stable. Three streams were selected for their moderate length, downstream environmental gradient, and the known presence of all three studied lineages. We sampled ten sites along the entirety of each stream during the spring, summer, and fall over two years. Mitochondrial 16S rDNA sequencing of 30 individuals per site was used to determine lineage composition. Preliminary results show a gradual change in dominance along and temporal stability between the spring season of two years in one of the streams. On the contrary, two other streams exhibited an irregular pattern, though this pattern was similar between them. So far, it seems that the environmental gradient of anthropogenic pressure is not the key driver of Gammarus<\/i> lineage distribution in the studied area, but more data is\u00a0needed to support or reject this conclusion. As our research progresses, it will enable us to compare the dynamic environment of streams with more stable habitat of spring fens, where alternative food source use and body size differences seem to promote the\u00a0syntopy of Gammarus<\/i> lineages. Interestingly, we did not detect body size differentiation among syntopic lineages in the streams. This may indicate that the lineage syntopy is driven by alternative mechanisms in different environments.<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”13:40 Bielikova O: Unveiling mitogenome of diving beetle Agabus bipustulatus (Linnaeus, 1767) using long-read nanopore sequencing” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” custom_margin=”||5px||false|false” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n Recent advances in DNA sequencing techniques have greatly accelerated the\u00a0acquisition of large-scale data. Among others, access to the information on\u00a0complete mitogenomes has been greatly facilitated, improving the resolution of\u00a0phylogenetic or other analyses requiring robust and reliable DNA data. Among the\u00a0most promising technology in this regard is long-read nanopore sequencing, developed by Oxford Nanopore Technologies. This study aims to elucidate the\u00a0composition features of the mitogenome of A.\u00a0bipustulatus<\/i>, achieved through long-read nanopore sequencing.\u00a0<\/span><\/span><\/p>\n Samples were collected from the Tatra Mountain glacial lakes (Western Carpathians, Slovakia), and High-Molecular-Weight DNA was utilised for\u00a0library preparation. Sequencing was performed using the Ligation sequencing gDNA \u2013 Native Barcoding Kit\u00a096 V14 (SQK-NBD114.96). Bioinformatic processing of reads for mitogenome generation involved several programs, including Flye, MitoFinder, Minimap2, Medaka and Mitos2. Nucleotide composition was analysed using MegaX, and skewness was calculated utilising standard formulae. Start and\u00a0stop codons were determined using exPasy. The resulting mitogenome was 17,876 base pairs (bp) long, with sequencing data achieving 139\u00d7 coverage. Among the 13 protein-coding genes (PCGs), three (nad6, nad3, nad1<\/i>) initiated with an ATA start codon, five (cox2, atp6, cox3, cob, nad4<\/i>) with an ATG start codon, four (nad2, atp8, nad5, nad4l<\/i>) with an ATT start codon, while the\u00a0cox1 <\/i>gene commenced with a CCG. Codon usage analysis revealed Leu <\/i>and Asn<\/i> as\u00a0the most frequent, while Cys <\/i>and Arg<\/i> were the least common. The stop codon TAA predominated among PCGs, with only 3 (nad3, nad1, cob<\/i>) ending with TAG. The overall base composition of the\u00a013\u00a0PCGs, which had a total length of 11,181\u00a0bp, was 34.40% for A, 43.56% for T, 11.58% for G, and 10.48% for C. The total length of tRNAs was 1,460\u00a0bp, with individual lengths ranging from 63 to 71\u00a0bp (the overall base composition of tRNAs was A: 39.66%, T: 38.91%, G: 11.58%, C: 9.74%). The total length of the 2 rRNAs was 2049\u00a0bp, with an overall base composition of 38.54% A, 43.72% T, 11.47% G, and 6.31% C. The length of the control region was 2,955\u00a0bp (A: 49.60%, C: 3.90%, T: 42.80%, G: 3.70%). Skewness analysis revealed a positive AT skew in\u00a0the\u00a0control region (0.074) and tRNAs (0.010), while PCGs (-0.118) and rRNAs (-0.063) exhibited a negative AT skew. The GC skewness was negative in the control region (-0.026) and positive for PCGs (0.050), tRNAs (0.092), and rRNAs (0.290). Compared to\u00a0previously published diving beetle mitogenomes, the sequence obtained by\u00a0nanopore-based long-read sequencing is significantly more reliable concerning problematic regions of mtDNA. Data obtained in this way can be a reliable basis for\u00a0subsequent comparative molecular studies, examining gene arrangement or\u00a0differences in\u00a0mitogenome size and composition. The study was supported by\u00a0the\u00a0European Union NextGenerationEU project No 09I03-03-V01-00075, titled \u201cScholarships for excellent researchers threatened by the conflict in Ukraine\u201c<\/i> under the\u00a0Recovery and Resilience Plan of the Slovak Republic, and by the Slovak National Grant Agency (VEGA), project no.\u00a02\/0084\/21.<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”14:00 Rimcheska B: Are semi-mountainous and mountainous benthic communities suitable for the delineation of the 6th (Eastern Balkan) and 7th (Hellenic Western Balkan) hydrofaunistic ecoregions?” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n According to the original concept of Illies (1978), adopted by the EU Water Framework Directive (WFD 2000), the areas of North Macedonia and Greece are divided longitudinally along the Vardar\/Axios River as the border between two ecoregions: Ecoregion 6 \u2013 Greek Western Balkans (right tributaries) and Ecoregion 7 \u2013 Eastern Balkans (left tributaries). To date, there are no macroinvertebrate data that would support an adjustment of the boundaries between the two hydrofaunal ecoregions for the area of North Macedonia. The aim of this study is an approach to a more realistic definition of the hydrofaunistic boundaries in the territory of North Macedonia, with respect to aquatic macroinvertebrate communities, based on the semi-mountainous and mountainous river types. To achieve this goal, a comparative analysis (PCA, Principal Component Analysis) of the macroinvertebrate communities from the studied areas (94 sites from eight river basins belonging to the three ecoregions 5, 6 and 7) was performed to verify their affiliation to a specific ecoregion. The results showed that the\u00a0strict delineation of hydrofaunistic boundaries between the studied ecoregions is\u00a0complex, mainly due to the peculiarities in the geographical distribution of\u00a0macroinvertebrates and the biotypic characteristics of the rivers themselves, which show greater similarities in the ecoregions’ border zones. As the selected study sites from all three ecoregions were chosen based on their categorisation as semi-mountainous and mountainous river types, one would expect greater similarity between areas of similar elevation and substrate. Regarding the distribution of the studied watersheds of the selected semi-mountainous and mountainous rivers, the obtained results indicate the shift of the border to the west, where the entire Vardar watershed belongs to Ecoregion 6 \u2013 Greek Western Balkans. These results would be a good basis for future research in relation to the other biological quality elements in order to find the best approach to support the proposed delineation between ecoregions 6 and 7.<\/span><\/p>\n This study was funded by \u201cProgramme for Support of Young Researchers and PhD Students at the Bulgarian Academy of Sciences\u201d (Grant No. DFNP-17-108\/28.07.2017 \u2013 \u201cImplementation of biotic indices BMWP and ASPT in order to evaluate the ecological status of mountain and semi-mountain rivers in the 7th Ecoregion (Eastern Balkans)\u201d) and by the \u201cJapan Funds-in-Trust Project\u201d (REF.: ERI\/MSP\/PPF\/LZF\/15.037) \u2013 \u201cManagement of freshwater bodies with implementation of EU-Water Framework Directives using aquatic macroinvertebrates as the necessary measure for sustainable development of freshwater ecosystems in less developed countries\u201d.<\/span><\/p>\n [\/et_pb_accordion_item][\/et_pb_accordion][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_accordion icon_color=”#0C71C3″ use_icon_font_size=”on” icon_font_size=”25px” _builder_version=”4.25.2″ _module_preset=”default” width=”100%” max_width=”100%” module_alignment=”center” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][et_pb_accordion_item title=”SS2: RESPONSE TO DRYING 2″ open=”on” _builder_version=”4.25.2″ _module_preset=”default” body_font=”|600|on||||||” body_text_align=”left” custom_margin=”||5px||false|false” global_colors_info=”{}” toggle_text_color=”#0C71C3″ toggle_level=”h5″ toggle_font=”|700|||||||” toggle_text_align=”left” toggle_font_size=”20px”]<\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”13:00 Straka M: Disentangling environmental drivers of macroinvertebrate community structure: the role of stream drying and wastewater pollution” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” custom_margin=”||5px||false|false” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n Due to the ongoing climate change bringing long-term deficits of precipitation together with rising evapotranspiration related to higher temperatures, there is\u00a0an\u00a0increasing proportion of intermittent streams in continental temperate zone. Drying streams are exposed to diverse anthropogenic impacts including the input of\u00a0urban pollution. The outflow of wastewater from sewage treatment plants are one of\u00a0the most common forms of organic pollution in stream ecosystems and has adverse effect on benthic invertebrates. The response of stream biota to increased saprobity is\u00a0well described in\u00a0perennial systems. However, the evidence of structural and\u00a0functional changes in\u00a0benthic invertebrate assemblages in polluted intermittent streams is scarce.<\/span><\/p>\n Using the \u201creal world experiment\u201d with sampling sites up- and downstream of sources of wastewater pollution in intermittent\/perennial streams we studied how both factors affect benthic invertebrate community. We analysed water chemistry, duration of dry period and benthic invertebrate communities within the dataset of 16 sites from the Czech Republic. Benthic invertebrate community clearly differed among the four studied groups (perennial non-polluted, perennial polluted, intermittent non-polluted, intermittent polluted) and was organised along two gradients. These gradients represent both studied factors (pollution, intermittence) and our results suggest that both factors have distinct but not the same effect on benthic invertebrates. Project was supported by the Czech Science Foundation (GA23-05268S) and H2020 project DRYvER (869226).<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”13:20 Szeles J: The effects of drought and habitat degradation on environmental filtering and limiting similarity” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” custom_margin=”||5px||false|false” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n Drought and the deterioration of habitats can happen independently or at the same time, impacting the presence of specific characteristics in groups of stream-dwelling invertebrates. Within the context of limiting similarity theory, we explore whether environmental filtering or limiting similarity functions as the main influencing factor under three different environmental conditions: unstressed (where there is constant water flow and a good ecological potential), single-stressed (due to drought or poor ecological potential), and multi-stressed environments (affected by both drought and\u00a0poor ecological potential). The traits of macroinvertebrates were monitored monthly, from March to December 2020, two trait databases of macroinvertebrates were used (DISPERSE and Freshwater Ecology) for trait analyses. Our results strongly support the idea that in both unstressed and single-stressed environments, limiting similarity is the primary factor shaping the structure of the macroinvertebrate community. It’s not just the quantity of stress, but rather its quality, that amplifies the\u00a0impact of environmental filtering. The drought has a greater impact on the aquatic ecosystem compared to habitat degradation as it eradicates living conditions when water dries up, whereas poor habitat conditions only limit them. Our research identifies universally beneficial trait states that remain consistent across different stressors, alongside specific trait states that exhibit unique responses to each type of stress. This study offers valuable perspectives for the management of freshwater habitats and\u00a0the\u00a0development of adaptive conservation strategies in light of the rising global frequency of droughts.<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”13:40 M\u00f3ra A: No way back: strong negative effects of stream intermittence on dragonfly (Odonata) assemblages” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” custom_margin=”||5px||false|false” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n In the last decades the proportion of intermittent rivers and streams has significantly increased as a consequence of climate change, affecting macroinvertebrate assemblages. Among them, dragonflies are usually top predators in stream ecosystems and any change in their assemblages might influence the whole community. Although they are considered potentially less affected by flow intermittence due to their strong dispersal abilities, high fecundity and high temperature tolerances, our knowledge on the effect of intermittency on Odonata is still limited. In the framework of the DRYvER project (Horizon2020 #869226) stream-dwelling Odonata were studied in three ecoregions of Europe differing in climate and intensity of intermittency: in the Boreal ecoregion, with a cold temperate climate and in general no flow intermittence (Finland); in the Pannonian ecoregion, with continental climate, and where the flow intermittence is a relatively new phenomenon (Hungary), and in the Mediterranean ecoregion, with Mediterranean climate and seasonal flow intermittence (Spain). Quantitative samples were taken bimonthly through six sampling campaigns in 2021\/2022. Odonata larvae were identified at species level and their body length was measured. Dragonfly larvae were almost absent from intermittent sections, even if surviving might have been possible in remaining pools. In cases when Odonata larvae occurred in intermittent sections, the number of species and number of individuals, and therefore their diversity were significantly lower than in permanent sections. Differences were detected in the size distribution of larvae between permanent and intermittent sections, but it was strongly species-specific depending on the life history of the species. The changes in size distribution showed temporal variability, i.e., the size of the larvae was significantly smaller after drying up in intermittent streams, indicating the occurrence of only newly hatched larvae. Our results demonstrate a remarkable negative effect of intermittency on dragonfly assemblages. Although some species would be able to re-colonise the rewetted sections, their long larval development prevents them from establishing a viable population in these sections.<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”14:00 Boz\u00f3ki T: Resistance, not resilience traits, structure macroinvertebrate communities in newly drying stream sections” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n Transitioning from perennial to non-perennial flow regimes causes ecological shifts in aquatic communities. Aquatic macroinvertebrates deploy resistance and resilience strategies to cope with flow intermittency, crucial in rivers with long-term seasonal dry episodes. Less is known, about how these strategies support community persistence in streams that only recently have experienced drying, and where local assemblages lack such adaptations. Our study conducted two four-season campaigns, separated by a one-year break, to assess macroinvertebrate responses in newly drying intermittent streams by comparing intermittent and perennial stream sections. We characterise communities from structural and functional perspectives, and then evaluate the response at the trait state level. We observed a decline in taxa richness and abundance, but not structural diversity, in response to flow intermittency. Resistance traits are more important than resilience traits in structuring macroinvertebrate communities in newly intermittent stream sections. Taxa in intermittent sections exhibit a smaller trait space, indicating lower functional redundancy. The macroinvertebrate response to intermittency lacks a predictable pattern, suggesting time-dependent and trait-state-specific colonisation by adapted taxa and community assembly with resistance and resilience strategies. As river drought increases due to climate change, recognising the temporal dimension becomes crucial for understanding ecological responses to intermittency.<\/span><\/p>\n [\/et_pb_accordion_item][\/et_pb_accordion][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.16″ custom_padding=”10px|0px|39px|0px||” border_color_all=”#4646c4″ global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_accordion icon_color=”#0C71C3″ use_icon_font_size=”on” icon_font_size=”25px” _builder_version=”4.25.2″ _module_preset=”default” width=”100%” max_width=”100%” module_alignment=”center” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][et_pb_accordion_item title=”RS1: DIVERSITY – BIOGEOGRAPHY – PHYLOGEOGRAPHY 3″ open=”on” _builder_version=”4.25.2″ _module_preset=”default” body_font=”|600|on||||||” body_text_align=”left” custom_margin=”||5px||false|false” global_colors_info=”{}” toggle_text_color=”#0C71C3″ toggle_level=”h5″ toggle_font=”|700|||||||” toggle_text_align=”left” toggle_font_size=”20px”]<\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”15:00 Weber D: Revised overview of the subterranean aquatic fauna of Luxembourg” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” custom_margin=”||5px||false|false” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n The Grand Duchy of Luxembourg is located in the transition area between Western and Central Europe. A team has been established at the National Museum of Natural History Luxembourg that focuses on recording subterranean life. The project \u201cScientific findings, basic data collection and monitoring of subterranean habitats in the Grand Duchy of\u00a0Luxembourg\u201d focuses on recording all plant, fungal and animal species living in\u00a0underground environments. Luxembourg is rich in natural caves but also artificial cavities, e.g., mines for iron ore, copper, antimony, slate, gypsum, dolomite. Also, subterranean military installations such as the casemates of the City of Luxembourg comprise prominent objects. However, running or standing waters in underground environments being directly human-accessible are scarce. Therefore, groundwater measuring points and shaft wells are sampled, in addition to springs as outflows of groundwater. Interesting results were also obtained from the investigation of interstitials close to riverine shorelines.<\/span><\/p>\n Specimens were collected mainly by sieving with four sieves with mesh sizes of 5,000\u00a0\u03bcm, 1,000\u00a0\u03bcm, 600\u00a0\u03bcm and 200\u00a0\u03bcm. Meat-baited tin cans were placed in mines. Numerous experts carry out the morphological identification of various animal groups. Questionable results and species thought to be new to science are genetically analysed in the museum’s own laboratories.<\/span><\/p>\n An overview of the proven classes and orders is given. As can be expected, the focus of subterranean aquatic fauna is on crustaceans. Ostracods (12 species) and copepods (27 species) are frequently found. The malacostracans are represented by the orders of isopods (two species) and amphipods, the latter by the families Crangonyctidae (one species), Pseudoniphargidae (one species), and Niphargidae (eight species). Two Niphargus<\/i> species are new to science. They will be described in 2024 and one even bears the name of the Grand Duchy.<\/span><\/p>\n The faunistic outcomes from caves are used for the Habitats Directive assessment of the Habitat type 8310. In addition, artificial cavities with an unusual species inventory are protected, such as the slate mine Laura in Haut-Martelange in which Niphargus boulangei<\/i> was found has already been categorised as being of high conservation value. So far, only one locality of this species (northern France), which has probably been destroyed in the interim, has been published. In the meanwhile, there are sufficient records to analyse them biogeographically, then in connection with neighbouring regions. Furthermore, previously neglected biotope groups, such as castle wells or groundwater measuring points, are to be intensively investigated. We also hope that new collection techniques such as downpipes, net collectors and wood washing will yield previously unknown results.<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”15:20 Salussolia A: From darkness to light: molecular phylogenetic analysis of the amphipod Niphargus elegans reveals repeated colonization of epigean environments from groundwaters” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” custom_margin=”||5px||false|false” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n Niphargus elegans<\/i> Garbini, 1894 is one of the few surface species in the predominantly groundwater genus Niphargus<\/i>. Described on material from the surroundings of Verona, Italy, it shows a wide distribution in the Padanian Plain and along the Adriatic coast where it inhabits surface streams, rivers, and ponds. As for many niphargid species, the\u00a0taxonomy of N.\u00a0elegans<\/i> is intricate. Considered by some authors as a subspecies of\u00a0the central-European N.\u00a0puteanus<\/i> (Koch, 1836), it was later enlarged to include spring-dwelling subspecies in Slovenia (N.\u00a0illidzensis slovenicus <\/i>S. Karaman, 1932, subsequently transferred to N.\u00a0elegans<\/i> by Gordan Karaman) and Croatia (N.\u00a0elegans zagrebensis<\/i> S.\u00a0Karaman, 1950); furthermore, it was reported from springs and caves in Romania.<\/span><\/p>\n We conducted a DNA-based species delimitation using four molecular markers (two nuclear rRNA gene fragments \u2013 ITS and 28S, the nuclear histone H3 gene and a fragment of the mitochondrial COI) to investigate the relationships among the alleged N.\u00a0elegans<\/i> subspecies as well with Italian and Romanian populations attributed to N.\u00a0elegans<\/i>. A\u00a0phylogenetic analysis based on a concatenated dataset of four markers was also performed to investigate the relationships between N.\u00a0elegans<\/i> and two other surface niphargids, N.\u00a0valachicus <\/i>Dobreanu & Manolache, 1933 and N.\u00a0hrabei<\/i> Karaman, 1932, both widely distributed in the Danubian basin in western Europe. We tested (i) whether Niphargus elegans<\/i> is monophyletic and whether its alleged subspecies are actually distinct species, (ii) whether its phylogenetic position suggests a secondary colonisation of the epigean environment and, in that case, (iii) whether N.\u00a0elegans<\/i>, N.\u00a0hrabei<\/i> and\u00a0N.\u00a0valachicus<\/i> independently colonised surface waters from groundwaters.<\/span><\/p>\n Results show that despite their morphological similarities, the three N.\u00a0elegans<\/i> populations from Italy, Slovenia, and Croatia are three distinct, closely related species. By\u00a0contrast, Romanian populations previously called N.\u00a0elegans<\/i> belong to a different clade (close to N.\u00a0valachicus<\/i>) that is a complex of new or poorly described species, including N.\u00a0pater<\/i> Mehely, 1941. Italian N.\u00a0elegans<\/i>, N.\u00a0hrabei<\/i> and N.\u00a0valachicus<\/i>, despite their ecological similarity, are not closely related and independently colonised surface waters from three different groundwater ancestors. These results highlight that although the genus Niphargus<\/i> shows major adaptations to groundwater life (such as eye and pigment loss), it repeatedly colonised surface waters in different geographical areas in\u00a0Europe.<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”15:40 Prati S: Neocaridina davidi, a feral shrimp in central Europe: range expansion and hidden parasites” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” custom_margin=”||5px||false|false” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n The release of ornamental pets and associated pathogens outside their native range might directly or indirectly impact the recipient community. In central Europe, feral freshwater species originating in tropical and sub-tropical regions are typically restricted to thermally altered environments such as heated waters and thermal springs. However, species like the\u00a0shrimp Neocaridina davidi<\/i> have high environmental plasticity and may acclimate to\u00a0cooler temperatures. A widening thermal niche may eventually overcome thermal barriers, further expanding the range, and enhancing transmission opportunities for host generalist parasites. This study investigates the observed (field observations) and theoretical (species distribution models) range expansion of N.\u00a0davidi<\/i> and associated parasites across Europe. We report the establishment of three new N.\u00a0davidi<\/i> populations in thermally altered waters in Germany, Hungary, and Slovakia, and a range expansion into colder environments. Species distribution models predict suitable thermal habitats in\u00a0Mediterranean regions, with a foreseeable expansion towards Western Europe and\u00a0the\u00a0Balkans by 2050.<\/span><\/p>\n Furthermore, we confirm the presence of the microsporidian parasite Ecytonucleospora hepatopenaei<\/i> in feral N.\u00a0davidi<\/i> populations throughout Europe and expand the list of\u00a0microsporidians found in this host from two to four. We present the first evidence of\u00a0parasite transmission between N.\u00a0davidi <\/i>and the invasive crayfish Procambarus clarkii<\/i>, indicating a potential for parasite exchange with native fauna. Such possibility, coupled with an ongoing range expansion of N.\u00a0davidi<\/i> bolstered by human-mediated introductions and\u00a0climate change, will likely exacerbate the impact on native biota.<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”16:00 Weber D: First genetically confirmed discovery of Niphargus (Malacostraca: Niphargidae) in Germany north of the Ice Age glaciation” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n Niphargids (Malacostraca: Niphargidae: Niphargus\u00a0<\/i>sp.) constitute a family of\u00a0crustaceans, with over 450 species described to date, predominantly inhabiting subterranean environments. As a result, they exhibit cavernicolous adaptations, including depigmentation and eyelessness. These crustaceans are widespread, ranging from Spain to Iran, with approximately 30 species identified in Germany. However, evidence of their occurrence north of the glacial boundary from the last ice age glaciations is scarce. Previous records from the United Kingdom, the Netherlands, and\u00a0Poland either turned out to be misidentified or lacked sufficient recollection.<\/span><\/p>\n This study presents, for the first time, DNA barcoding confirmed records of Niphargus<\/i> from three German springs, specifically rheocrenes, north of the former glaciation, situated approximately 70\u00a0km north of the glacier edge associated with the Elster and\u00a0Saale glaciations. These springs are approximately 60 kilometres apart from each other.<\/span><\/p>\n All three springs harbour Niphargus aquilex<\/i>, a species widely distributed from England to\u00a0northern France, Belgium, Luxembourg, and Germany, albeit not commonly found. The Folmer fragment of the Cytochrome c Oxidase Subunit 1 (COI) gene was sequenced for 19 individuals, revealing two haplotypes. The more common haplotype is also present in\u00a0the\u00a0southern distribution area of the species, providing evidence of\u00a0post-glacial migration to the north. Contrary to initial assumptions, the species did not survive the ice age in\u00a0nunataks or under the ice sheet.<\/span><\/p>\n The new findings, however, prompt several questions. Although 18 of the 19 sequences are identical and match sequences found in the south, one sequence is missing in\u00a0the\u00a0south. Could this indicate a population that survived under the ice? Alternatively, is it a recent mutation involving at least two base pairs within the presumed 100,000 years? The\u00a0N.\u00a0aquilex<\/i> group is a species complex comprising 10 to 20 species, the\u00a0majority of\u00a0which are cryptic, while the rest are pseudocryptic. Why, then, has only one species from this complex migrated northward? Unlike other species (e.g., Niphargus puteanus, Niphargus fontanus<\/i>), which have migrated northwards parallel to\u00a0the rivers, N.\u00a0aquilex’s<\/i> colonisation of the north poses unique questions about its adaptive mechanisms.<\/span><\/p>\n [\/et_pb_accordion_item][\/et_pb_accordion][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_accordion icon_color=”#0C71C3″ use_icon_font_size=”on” icon_font_size=”25px” _builder_version=”4.25.2″ _module_preset=”default” width=”100%” max_width=”100%” module_alignment=”center” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][et_pb_accordion_item title=”SS2: RESPONSE TO DRYING 3″ open=”on” _builder_version=”4.25.2″ _module_preset=”default” body_font=”|600|on||||||” body_text_align=”left” custom_margin=”||5px||false|false” global_colors_info=”{}” toggle_text_color=”#0C71C3″ toggle_level=”h5″ toggle_font=”|700|||||||” toggle_text_align=”left” toggle_font_size=”20px”]<\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”15:00 Polovi\u0107 L: Comprehensive database of drying resistance and resilience traits from drying rivers across Europe” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” custom_margin=”||5px||false|false” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n Drying River Networks (DRNs) are highly dependent on the dynamics of local climate conditions, which greatly influence the flow regime, the duration of dry and wet periods and\u00a0the frequency of drying events. As such, DRNs are one of the most vulnerable ecosystems to climate change and anthropogenic exploitation. In order to secure biodiversity, functional integrity and ecosystem services in drying river networks, intricate ecological dynamics governing these ecosystems need to be unravelled. In this effort, the\u00a0trait-based approach is utilised to emphasise the significance of functional biodiversity in sustaining ecosystem processes under environmental stressors and to identify in which measure each functional trait exhibits resistance or vulnerability to drying events.<\/span><\/p>\n A part of that effort is building a comprehensive database containing information on\u00a0drying resistance (ability to endure) and resilience (ability to recover) traits for\u00a0freshwater bacteria, fungi, diatoms, macroinvertebrates, and fishes. Through collaborating with experts and\u00a0studying literature, a list of traits relevant to drying event survival was compiled for each of the five biotas. Each trait has been categorised, coded and its function described, and freshwater community information was obtained during the sampling process for project DRYvER. Subsequently, the database was compiled assigning trait values to listed taxa using readily available data from existing databases and published literature. The database’s development involved rigorous collection, cleaning, standardisation, and harmonisation of\u00a0trait data to ensure the thorough representation and precision of trait variability across biota. Using the database, trade-off analysis was conducted.<\/span><\/p>\n Construction of the database is a continuous process as newly described trait data is\u00a0appearing and newly developed technologies offer better solutions for higher levels of\u00a0precision. Through collation and further analysis of trait data, the trait database enhances our understanding of the ecological mechanisms underpinning DRN resilience which contributes to better understanding of the effects of climate change on freshwater biodiversity across Europe.<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”15:20 Szloboda A: The resilience of stream food webs to drying: insights from European drying river networks” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” custom_margin=”||5px||false|false” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n While intermittent rivers have long been common in southern regions of Europe, global change is currently prolonging dry periods and causing intermittent flow in formerly perennial rivers, even in northern regions of Europe. The consequences of drying for the\u00a0structure and productivity of the food webs in these systems are still unknown. Here, we aimed to investigate the stability of river food webs in the face of drying. We sampled six Drying River Networks (DRNs) across six European countries (Croatia, Czechia, France, Finland, Hungary, and Spain) within the EU-funded DRYvER project (869226). These DRNs varied along a latitudinal gradient and in terms of their historical exposure to intermittent flow. We sampled aquatic macroinvertebrate communities and assessed hydrological conditions every two months for one year. We studied taxon-specific biomass using image analysis and estimated secondary productivity from biomass and water temperature data. We found that (1) intermittent branches of river systems were less productive than perennial branches, and (2) DRNs in more southern regions exhibited lower declines in secondary productivity compared to DRNs experiencing drying recently. Crustacea and Diptera were identified as the primary contributors to secondary productivity, but different orders of invertebrates contributed to productivity in different DRNs and within intermittent and perennial sections. We discuss our findings in light of\u00a0the future of river networks in Europe under expected climate change.<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”15:40 V\u00e1rb\u00edr\u00f3 G: Drought driven directional changes in presence-absence macroinvertebrates community metrics” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” custom_margin=”||5px||false|false” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n Understanding the influence of environmental stressors on community dynamics is\u00a0essential for evaluating ecosystem resilience and conserving biodiversity. In this investigation, we focus on aquatic macroinvertebrates as indicators of freshwater ecosystem health in order to assess the effects of drought conditions on presence-absence community metrics. Beta diversity encompasses both community composition and species turnover, reflecting non-directional and directional changes, respectively. Despite various methods for partitioning variation and assessing community-level phenomena, approaches to evaluating species turnover along temporal gradients based on a unified conceptual framework are currently limited. In our study, we explore the possibility of examining different aspects of directional changes along a temporal gradient using presence-absence community data. We define a spectrum of turnover and nestedness concepts by integrating measures of community overlap, species loss, and gain across sampling units while developing functions for their quantification. These concepts function as indicators of ecological phenomena with measures derived from raw species counts as well as relativized forms such as the Jaccard and S\u00f8rensen indices. The results highlight that actual communities display directional responses to\u00a0specific ecological gradients, thus shedding light on previously overlooked aspects within the field of ecology.<\/span><\/p>\n By elucidating how presence-absence community metrics respond under drought conditions, our study contributes towards gaining greater insights into how environmental stressors shape freshwater ecosystems, crucial knowledge necessary for formulating conservation strategies aimed at mitigating climate change impacts on\u00a0aquatic biodiversity management practices.<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”16:00 Bo\u00f3z B: Flow intermittence changes the occupancy frequency distribution of stream chironomid assemblages” _builder_version=”4.25.2″ _module_preset=”default” body_font=”||||||||” body_font_size=”16px” hover_enabled=”0″ global_colors_info=”{}” toggle_font=”–et_global_heading_font|500|||||||” toggle_font_size=”18px” open=”off” sticky_enabled=”0″]<\/p>\n In community ecology research, the characterisation of species occupancy frequency distribution (OFD) patterns has been proven to be a suitable method to understand the\u00a0mechanisms underlying the organisation of the communities on a regional scale. OFDs provide information on the relative dominance of rare and common species in\u00a0the\u00a0communities by multiple shapes of histograms based on the occurrence frequencies of the species. Among them, unimodal (characterised by rare species) and\u00a0bimodal (characterised by additional common species) patterns might indicate well-structured communities, organised by various local and regional filters. In the case of\u00a0passive dispersers, like Chironomidae, a right-skewed unimodal OFD pattern is\u00a0expected in undisturbed conditions at a regional scale. We studied the OFD pattern of\u00a0stream-dwelling chironomid assemblages in drying river networks (DRNs) suffering from various degrees of intermittency. We assumed that flow intermittency, as an\u00a0environmental disturbance, can cause changes in the organisation of the assemblages. Quantitative samples were taken bimonthly within the framework of the DRYvER project (Horizon2020 #869226) through six sampling campaigns in 2021. Chironomid larvae were identified morphologically to the lowest possible taxonomic level (targeting species level) from Hungarian, Croatian and Czech DRNs. In contrast with our expectations, in\u00a0the\u00a0permanent sites, the chironomid assemblages showed bimodal OFD pattern characterised by many rare, relatively few moderately frequent and several common species. On the contrary, the assemblages in intermittent streams were characterised by\u00a0a\u00a0rather right-skewed unimodal OFD pattern, with a high number of rare species but few common species, and relatively high proportion of moderately frequent species. Species that were common in permanent streams become moderately frequent or rare in\u00a0intermittent streams. It suggests that previously suitable habitat decreased in the area due to flow intermittence. Our results demonstrate that drying events can have a\u00a0remarkable influence on the processes that shape the chironomid assemblages at\u00a0a\u00a0regional scale.<\/span><\/p>\n [\/et_pb_accordion_item][\/et_pb_accordion][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.25.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.25.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.25.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.25.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_toggle title=”16:40 PRESENTATION & WORKSHOP” open_toggle_text_color=”#0C71C3″ closed_toggle_text_color=”#0C71C3″ icon_color=”#313476″ use_icon_font_size=”on” icon_font_size=”25px” open_icon_color=”#313476″ open_use_icon_font_size=”on” open_icon_font_size=”25px” _builder_version=”4.25.2″ title_text_color=”#0C71C3″ title_font=”|600|||||||” title_text_align=”center” title_font_size=”22px” closed_title_font=”||||||||” closed_title_text_align=”center” closed_title_line_height=”1.8em” body_font_size=”20px” body_line_height=”1.8em” background_color=”#f5f9fb” text_orientation=”center” custom_margin=”-63px||||false|false” custom_padding=”||29px|||” global_colors_info=”{}”]<\/p>\n All participants are welcome in the Special presentation & Workshop at Monday evening. Both the presentation and the workshop will be provided by the conference partner, the\u00a0NGS GEEKS Division of I.T.A.-Intertact, s.r.o., which states that “Every month there are many innovations in NGS. Some take our breath away, others make us laugh out loud.\u201d<\/i><\/span><\/span><\/p>\n Presentation: Technologies for Advanced Biology Research<\/i><\/strong><\/p>\n The development of various approaches to studying biology using specific methods for\u00a0NGS library preparation is so rapid that not many can follow up. Therefore, we offer a\u00a0pre-selected overview of technologies that are, in our opinion, of high interest to\u00a0experimental field biologists, solving the most burning issues such as (i) insufficient amount of sample, (ii) detailed analysis of genome and transcriptome, (iii) low quality of\u00a0nucleic acid, (iv) portable instrumentation for qPCR and sequencing, or (v) user-friendly sample storage management.\u00a0<\/span><\/span><\/span><\/p>\n Workshop: Nanopore Sequencing for Biologists<\/i><\/strong><\/p>\n The workshop’s scope is to educate participants on Oxford Nanopore sequencing technology and the manual work necessary to create a sequencing library and launch sequencing experiments. Short part will be dedicated to sequencing experiment QC data analysis in EPI2ME analysis software.<\/span><\/p>\n [\/et_pb_toggle][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.24.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.24.2″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”https:\/\/www.aquabol.sk\/cesamir2020\/wp-content\/uploads\/2024\/06\/CESAMIR2024_SUMMARY-sun-mon.jpg” title_text=”CESAMIR2024_SUMMARY sun-mon” _builder_version=”4.24.2″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ admin_label=”Subscribe” _builder_version=”4.16″ background_color=”#f5f9fb” custom_padding=”50px|0px|50px|0px||” animation_style=”slide” animation_direction=”bottom” animation_intensity_slide=”20%” animation_starting_opacity=”100%” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_signup mailchimp_list=”Mailchimp|fe69e960a2″ first_name_field=”off” last_name_field=”off” success_message=”Thank you!” title=”Stay Up To Date” description=”<\/p>\n Subscribe to our mailing list and keep informed with all the news about CESAMIR2024 conference! *<\/p>\n * Your e-mail address will be used for conference purposes only.<\/p>\n ” _builder_version=”4.23.4″ form_field_background_color=”#003c7c” form_field_text_color=”#ffffff” form_field_focus_background_color=”#282b66″ form_field_focus_text_color=”#ffffff” header_font=”|600|||||||” header_font_size=”35px” header_line_height=”1.3em” body_font=”||||||||” body_font_size=”18px” body_line_height=”1.8em” result_message_font=”||||||||” result_message_text_color=”#000000″ result_message_font_size=”30px” use_background_color=”off” custom_button=”on” button_text_size=”14px” button_text_color=”#ffffff” button_bg_color=”#005fba” button_border_width=”8px” button_border_color=”#005fba” button_border_radius=”0px” button_letter_spacing=”1px” button_font=”|700||on|||||” button_icon=”=||divi||400″ background_layout=”light” border_radii_fields=”on||||” button_letter_spacing_hover=”1px” focus_background_color=”#ffffff” focus_text_color=”#ffffff” global_colors_info=”{}” button_text_size__hover_enabled=”off” button_one_text_size__hover_enabled=”off” button_two_text_size__hover_enabled=”off” button_text_color__hover_enabled=”off” button_one_text_color__hover_enabled=”off” button_two_text_color__hover_enabled=”off” button_border_width__hover_enabled=”off” button_one_border_width__hover_enabled=”off” button_two_border_width__hover_enabled=”off” button_border_color__hover_enabled=”off” button_one_border_color__hover_enabled=”off” button_two_border_color__hover_enabled=”off” button_border_radius__hover_enabled=”off” button_one_border_radius__hover_enabled=”off” button_two_border_radius__hover_enabled=”off” button_letter_spacing__hover_enabled=”on” button_letter_spacing__hover=”1px” button_one_letter_spacing__hover_enabled=”off” button_two_letter_spacing__hover_enabled=”off” button_bg_color__hover_enabled=”off” button_one_bg_color__hover_enabled=”off” button_two_bg_color__hover_enabled=”off”][\/et_pb_signup][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":" Day 1 – Monday, July 08Freshwater ecosystems account for 0.3% of the planet’s freshwater but they are the\u00a0habitat for 9% of all described species and 35% of vertebrate species. The levels of\u00a0freshwater biodiversity loss are alarming, doubling those found in terrestrial or marine ecosystems. Mediterranean climate regions are considered global hotspots of\u00a0biodiversity, also for freshwater […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":484,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"yoast_head":"\nCongress Hall \u00a0 10:30 – 11:50<\/h4>\n
Business Lounge \u00a0 10:30 – 11:50<\/h4>\n
Congress Hall \u00a0 13:00 – 14:20<\/h4>\n
Business Lounge \u00a0 13:00 – 14:20<\/h4>\n
Congress Hall \u00a0 15:00 – 16:20<\/h4>\n
Business Lounge \u00a0 15:00 – 16:20<\/h4>\n